Cold applications for recovery in adolescent athletes: a systematic review and meta analysis
© Murray and Cardinale. 2015
Received: 16 April 2015
Accepted: 21 September 2015
Published: 12 October 2015
Recovery and regeneration modalities have been developed empirically over the years to help and support training programmes aimed at maximizing athletic performance. Professional athletes undergo numerous training sessions, characterized by differing modalities of varying volumes and intensities, with the aim of physiological adaptation leading to improved performance. Scientific support to athletes focuses on improving the chances of a training programme producing the largest adaptive response. In competition it is mainly targeted at maximizing the chances of optimal performance and recovery when high performance levels are required repeatedly in quick succession (e.g. heats/finals). In recent years, a lot of emphasis has been put on recovery modalities. In particular, emphasis has been placed on the need to reduce the delayed onset of muscle soreness (DOMS) typically evident following training and competitive activities inducing a certain degree of muscle damage. One of the most used recovery modalities consists of cold-water immersion and/or ice/cold applications to muscles affected by DOMS. While the scientific literature has provided a rationale for such modalities to reduce pain in athletes and recreationally active adults, it is doubtful if this rationale is appropriate to aid training with adolescent athletes. In particular, since these methods have been suggested to potentially impair the muscle remodeling process leading to muscle hypertrophy. While this debate is still active in the literature, many coaches adopt such practices in youth populations, simply transferring what they see in elite sportspeople directly; without questioning the rationale, safety or effectiveness as well as the potential for such activity to reduce the adaptive potential of skeletal muscle remodeling in adolescent athletes. The aim of this review was to assess the current knowledge base on the use of ice/cold applications for recovery purposes in adolescent athletes in order to provide useful guidelines for sports scientists, medical practitioners, physiotherapists and coaches working with such populations as well as developing research questions for further research activities in this area. Based on the current evidence, it seems clear that evidence for acute benefits of such interventions are scarce and more work is needed to ascertain the physiological implications on a pre or peri-pubertal population.
KeywordsRecovery Ice Cold Youth Adolescent Athlete Elite
The typical structure of any training programme aimed at enhancing human performance is based around the principle of overload. The most common example of a training programme consists of cycles of progressively intensified training separated by a drop in volume, intensity or both or recovery weeks across a season; this cyclical approach has been suggested to disrupt the physiologic homeostasis through training so that the performance capacity improves via supercompensation [1, 2]. To maximize the effectiveness of such a programme, there is the need to subject the athlete to not only periods of intensive training loads but crucially the appropriate recovery to facilitate adaptation .
Such an approach is used by coaches worldwide not only to train adult athletes, but also to develop athletic potential in adolescents1. Unlike adults, the evidence for overtraining in young athletes does not seem to be strong, probably limited by the lack of appropriate longitudinal studies . Muscle damage is one of the many well-known physiological effects of increased loading, in particular when resistance exercise and/or repeated eccentric actions are employed [5–9]. When considering training planning, the extent of the ‘damage’ can be placed on a continuum from excessive (injury) to the minimum dose needed to cause a training effect. In recent years, a lot of studies and empirical activities have been targeted to identify the best recovery strategies for the athlete and coach to reduce any negative implications of training loads to allow quicker recovery whilst maximizing adaptation. Despite the fact that recovery is recognized as a need when planning the training activities of an athlete, a paucity of data exists on the implications of various recovery activities. There is no definition of the most ‘appropriate’ modality, protocol and timing according to the level of the athlete and their training goals .
Recent work has highlighted that the aim of most recovery interventions is to return psychological , physiological [12, 13], and performance variables  to the ‘pre-exercise’ level or to baseline conditions identified in the absence of fatigue. There have been a number of reviews in recent years on the use of recovery modalities within elite adult sport. The main focus for these has been on therapies utilizing different temperature mediums; cold in the form of water immersion and whole body cryotherapy [15–18] and heat via saunas and water immersion [19, 20]. A recent meta-analysis has suggested a large effect of cooling after exercise on the ability to retain sprint performance but much lower effects on endurance or strength . In all cases, performances were most affected at 4 days following the exercise session/insult used to study the effectiveness of the recovery intervention. On average though, these effects seemed relatively small (Hedges effect size = 0.28). However, a more recent review still supported the idea that such interventions can useful enough for competitive athletes, provided ‘appropriate’ methodologies can be utilized . Appropriateness should refer to the aim and the expected outcome of an intervention. Despite the fact that cold-water immersion (CWI) seems to be an effective (and therefore appropriate) strategy to reduce delayed onset muscle soreness (DOMS), it does not seem effective in restoring strength and might have some potential to restore power  in adult athletes. However, while some justification seems to be warranted for the use of such modalities in elite athletes, there is a paucity of data on the rationale and effectiveness for younger ones be they elite or amateur.
As muscle damage is one of the well-known physiological effects of increased loading, exercise protocols capable of creating muscle damage have mostly been used as the model to understand the effectiveness of recovery modalities. The main rationale being the ability of such a stimulus to induce marked changes in the skeletal muscles of individuals unaccustomed to resistance and/or eccentric exercise; from alterations at the cellular level to observed reductions in indices of muscle function . The discomfort and soreness associated with muscle damage generally increases and peaks between 24 and 72 h following the exercise/training bout causing it, before subsiding (if there is no further training) over the next 5–7 days  as the injury is gradually repaired [24, 25]. When the training is repeated, a marked reduction in muscle soreness and an increase in inflammatory markers is observed and this phenomenon has been termed the repeated bout effect, indicating an adaptive response to the training stimulus [26–28]. It is nowadays accepted that while the exact mechanism is not yet elucidated  the trained or preconditioned individual  should not be prone to the same levels of muscle damage and associated symptoms typically observed in the untrained if they use similar exercises in the training regime . The objective of any inflammatory process, is to repair injury and restore tissue function , (for a review of the mechanisms involved in muscle damage see ). This is one of the reasons why coaches prescribe varied forms of exercise, were the intensity and volume of the activities and the modalities of contractile activity are such that they create micro-trauma to foster the remodeling of muscle tissue. This training approach is aimed at triggering an adaptive response such that the muscle is more resistant to damage and any damage that does occur is repaired at a faster rate. It is also clear that a relatively small insult will produce this adaptation [34–36]. However, as inflammation and pain reduce the ability to train or compete, various approaches are taken to reduce the symptoms.
In an attempt to reduce the negative symptoms of inflammation, recovery techniques have been mainly targeted to affect skeletal muscles’ temperature and blood flow [17, 37–44]. The most popular approach involves CWI, which combines the effects of cold and increase in hydrostatic pressure to trigger multiple physiological effects capable of reducing pain perception and facilitate recovery. Research on young athletes is somewhat limited with regards to both the adaptation to training and the effects of various recovery modalities due to methodological and ethical constraints. The limited evidence available advocates that pre-pubertal youths recover quicker than adults  mainly due to their lower relative power capabilities, but also due to a relative larger flexibility and muscle compliance which makes them less susceptible to muscle damage than their adult counterparts [46–49]. Recent work  has shown that muscle damage (and its symptoms) is a lot less in pre-adolescents than adolescents and adults when similar eccentric exercise protocols and loading patterns were applied. Furthermore, the few previous studies published in pre and post-pubertal populations have clearly presented that the extent of muscle damage in children is less than adults [51, 52].
Despite the scarcity of research studies in this field, coaches and parents sometimes train and recover adolescent athletes in the same way as they would with adult populations, mostly attracted by the popular headlines of lay press and/or replicating what they see in professional sport. Considering the lack of scientific consensus on this topic and the need to develop an evidence-based approach to recovery strategies in adolescent athletes, we aimed to review the current literature with a specific focus on cold applications to identify safe and effective interventions currently known, explore the potential adaptive mechanisms and suggest new avenues for research in this field.
The rationale for cold applications and training adaptations in adolescent athletes
Exercise, inflammation and muscle remodeling
After high intensity exercise there is typically an acute performance reduction due to mechanical and/or metabolic stresses [21, 53, 54]. Exercise that induces a large metabolic stress (such as endurance or interval training) contributes to an increase in reactive oxygen species generation (ROS; [24, 55]). ROS have been shown to determine many acute and chronic alterations in skeletal muscle mainly characterized by marked inflammatory responses (for a review see ).
While there are a number of papers in the literature involving adult athletes, limited information exist on adolescent athletes. Recent work proposed that the resting blood redox balance is well maintained in adolescent endurance athletes due to adaptation to exercise . Furthermore, Kabasakalis and colleagues suggested that children, involved in intensive swimming training, could improve their antioxidant capacity in the same manner as adult athletes . As previously stated, when damaging exercise activities are performed, the initial insult causes a mechanical disruption of the muscle fibers. Subsequent damage is linked to inflammatory processes over the next 24 h and to changes in excitation–contraction coupling within the muscle . The inflammation sees a proliferation of fluid, plasma proteins and inflammatory cells [59–61] which is thought to amplify the initial muscle injury through increased ROS release and activation of phospholipases and proteases at the injury site .
Within an adult population, the time course of neutrophil accumulation (the primary step in the inflammation cascade) varies from as little as 45 min after eccentric exercise  up to 5 h , dependent on both the type and intensity of exercise . The subsequent course of the inflammation process depends on the relative balance between increases in pro-inflammatory (interleukin [IL]-1, IL-6, tumor necrosis factor) and anti-inflammatory cytokines (IL-10), to produce a sufficient inflammatory response to adequately repair injured tissue without creating an excessive response [64, 65].
The majority of published studies in adult populations have reported increased concentrations of IL-6 in the blood after both prolonged endurance and eccentric exercise [65–69], suggesting its usefulness as a biomarker to understand inflammatory responses to exercise. Further work has also hypothesised a relationship of IL-6 with intensity and duration of exercise . In fact, it has been shown that the release in the bloodstream is higher after prolonged endurance rather than brief maximal exercise. After 12 h of exercise, intensity seems to be the key determinant whereas under 12 h, it is duration . Furthermore, training status may also affect the IL-6 response with well-trained runners presenting lower IL-6 concentrations than untrained ones [71, 72]. Yet there are conflicting reports in the literature as higher post exercise responses in IL-6 were also positively related to performance improvements in highly trained male rowers . It is almost impossible to define the timeline of IL-6 kinetics in the bloodstream due to the varied protocols and timelines in the literature. Despite the surge in interest in interleukins and intense/damaging exercise the response in adolescent athletic populations is somewhat limited. Volleyball training in elite adolescent athletes has been shown to determine marked inflammatory responses  characterized by marked increases in IL-6. Cycling exercise for 60 min at an intensity of 70 % of VO2 max was also shown to determine marked increases in leukocytes, lymphocytes and cytokines in healthy boys and girls aged 12–14 . Tennis practice was also shown to determine marked inflammatory responses in healthy adolescents regularly engaged in tennis training (more than 17 h per week) . Finally, acute bouts of wrestling  performed by high school athletes were capable of causing an acute increase in pro-inflammatory cytokines after the sessions, as well as chronically over the course of the high school sporting season. This suggests that increased training and competition loads in contact sports can trigger significant inflammatory responses in young athletes . Limited studies exist on the consequences of eccentric resistance exercise in pre-, peri- and post-pubertal athletes.
Adolescents vs adults: differences in inflammation and muscle remodeling
Adolescent boys, similarly to adults, demonstrate enzymatic muscle changes specific to the modality of training employed similar to adults , albeit with lower magnitudes [80, 81]. This has been proposed as an explanation why recovery can be quicker than that of their adult counterparts . Despite the fact that adolescent athletes show similar adaptations to exercise as adults, they seem to not experience similar symptoms of muscle damage after intense exercise [46, 50, 51, 82]. Adequate training loading is necessary in young populations as excessive training volumes and intensities and inappropriate progressions in a young athlete can create overload and injury whilst the correct amount can promote growth and performance gains [83, 84].
Serum creatine kinase (CK) has been proposed as an indirect marker of muscle damage mainly because it can be determined with inexpensive assays and point of care testing. Although caution needs to be used as it is highly individual in its variability, its concentration in blood is affected by the training level and the muscle groups involved in exercise, to a greater extent than differences in exercise volume or intensity . In the sporting domain, most decisions regarding recovery interventions following intense training sessions are based on CK levels, however some caution needs to be applied when considering the implications for young athletes. In fact, the observed circulating levels of CK are typically lower in the young and elderly following exercise of the same relative intensity when compared to adults. Using CK as the unique marker to determine the need for an intervention to reduce inflammation may not represent the best informed decision in an adolescent group. In fact, Webber and colleagues investigated the CK response following a bout of downhill running, and observed a significantly greater CK increase in adults in comparison with prepubescent children . However, when body weight was accounted for, no significant age effects were observed, leading the authors to conclude that different increases in CK between adults and children were related to body weight. Other authors have reported that children are less susceptible to exercise-induced muscle damage than adults when performing bench press exercise , one leg–stepping exercise  plyometric exercise [48, 82] and resistance exercise performed with eccentric-type muscle actions .
The physiological meaning of increased serum CK following various forms of exercise has been recently reviewed  and consensus seems to be that increased levels following exercise may not always be indicative of muscle damage. Furthermore, CK levels cannot be used to ascertain the recovery from a bout of exercise, as it doesn’t necessarily relate to performance measures and it is evidently not highly sensitive in young athletes. In fact, the levels of CK induced by sporting activities  may induce myositis but are typically nowhere near the values recorded in case of rhabdomyolysis and up to 300 % lower than the increases observed following myocardial infarct  or in burn patients .
It has been shown that the activities of the anaerobic enzymes creatine kinase, adenylate kinase, and lactate dehydrogenase (LDH) in children are lower than in adults—possibly linked to the fact that children have a greater ability to oxidize lipids during exercise and have less reliance on anaerobic metabolism . Hence, if CK is used as a marker to ascertain the metabolic implications of training sessions in children and adolescents, it is likely that such an approach might not reflect the true extent of the effects of the training bout on skeletal muscle. The repeated bout effect observed in children and adolescents is also different than in adults. In fact, this occurs more readily to eccentric exercise in adolescent boys than adults  which may cloud any measurements made in a crossover design to assess the effectiveness of CWI.
Muscle fiber types should also be considered. Although there is some controversy regarding fiber type differences between children and adults [80, 91–93], there are only minor increases in the proportion of type II fibers with age [80, 91, 92]. Even with a greater proportion of type I muscle fibers in children, no strong correlation between fiber type composition and enzyme activity in skeletal muscle exists . Nonetheless we know that fast-twitch muscle fibers are the ones susceptible to muscle damage and there are a low number of them in adolescent groups . Muscle architecture differences between boys and adults should also be considered as previous work has postulated that a shift to a longer muscle length makes muscle less susceptible to damage [95, 96]. For these reasons, it seems evident that knowledge regarding muscle damage and remodeling in children and adolescents involved in routine, structured, sporting training is somewhat lacking. In particular, when it comes to the need of implementing recovery strategies, it is not clear if interventions like CWI might actually be detrimental to long-term developments.
Adolescent populations display reduced symptoms of muscle damage, perhaps in relation to a reduced nociceptive tolerance (i.e. they cannot work for as hard or as long or endure as high a relative level of pain). The perceived beneficial effects of CWI could also be due to placebo effects and expectation of a positive outcome. Adolescent athletes may experience the ‘pain’ of recovery as a challenge in which the perceived benefits are greater than the demands [97, 98]; the moderate pain of a cold immersion may be reported as pleasant and aid the placebo effect as its outcome is perceived as less painful than doing nothing . Building expectations that pain following training needs recovery interventions, as typically justified by coaches/parents might contribute to the positive effects perceived and prolonged unnecessary exposure can be sought due to peer pressure, which may be counterproductive and defeat the purpose of the intervention. Studies in young individuals have linked pain and guilt and participants with induced feelings of guilt were able to endure submersion of their hand in ice-water for longer compared to those who weren’t guilt ridden .
General physiological effects of cold applications
During immersion, the major heat exchange occurs via conduction with the surrounding water and hence the level of immersion is important as this dictates the body surface area in contact with the surrounding medium. Within an adult population as core body temperature decreases, so does heart rate  causing concomitant decreases in cardiac output [109, 110] and increases in peripheral resistance and arterial blood pressure . In order to preserve core temperature, the body alters blood flow, metabolism and oxygen consumption [109, 112] to make sure homeostasis is maintained.
Localised vasoconstriction reduces fluid diffusion into the interstitial space, which can assist in reducing muscle damage and acute inflammation. Typically the cooler the water, the greater the core-to-water temperature gradient and hence the rate of cooling . It may not be necessary to use very cold-water to reduce blood flow as a comparison of 8 and 22 °C CWI showed a 40 % reduction in both conditions . Skeletal muscle’s blood flow is not the only parameter affected by CWI. In fact, the neuromuscular system is also affected, as tissue cooling decreases the transmission rate along neurons by decreasing the production of acetylcholine . This has been shown to reduce both pain perception [116, 117] and muscle spasm [39, 118]. While this can be of benefit to recovery it must be considered in a practical context as cooling muscles can decrease muscular contractile speed and the force generating ability of the athlete directly after cold immersion [119, 120].
Within an adult cohort core temperature changes are dependent on a number of factors; the method of immersion , the duration [122, 123] and the adiposity of the individual involved [124, 125]. Regardless there is a relatively consistent pattern with skin temperature dropping rapidly in the first 3 min and reaching the minimum after 8-9 min . Superficial intramuscular temperature cools faster than deep muscle tissue with the magnitude of change being proportional to the thermal gradient . Given the typical duration of immersion in practical use the deeper tissues will reach a temperature minimum once cooling has actually ceased as heat continues to be lost to the superficial tissues surrounding them even after subjects leave the water [126–128]. This suggests what the athlete does immediately after immersion is important—modest cryotherapy (~20 °C) of a large mass may be successful if there is a period out of the water to allow this temperature ‘afterdrop’ to occur, i.e. the athlete doesn’t immediately leave the cold-water and have a hot shower or bath to ‘re-warm’. This may promote an intermittent or contrast-cooling technique as colder temperatures may be possible whilst limiting the potential for injury to superficial tissues. Alternatively this may promote a strategy of agitation of the immersion medium to prevent a ‘barrier layer’ forming around the subject and promote convective heat loss. Alternatively it may not be an issue in an adolescent population given their reduced adiposity, muscle mass and limb girth in comparison to an adult group. The timing of immersion in relation to exercise may also be important as high intensity exercise can create a greater thermal gradient.
Skeletal muscle metabolism
Enhanced metabolite clearance and reduced inorganic phosphate and hydrogen ion accumulation has been shown within skeletal muscle following CWI . Reduced muscle pH is a contributing factor to peripheral fatigue development and the subsequent reduction in its force-generating capacity . Cooling skeletal muscles has been shown to result in a reduced acidosis as measured by 31P magnetic resonance spectroscopy one hour after exercise . Any benefit of cold-induced pH restoration could be concealed by the reduced functional capacity of a cold muscle . Similarly, but with a different method (hyperoxia) Vanhatalo and colleagues showed that the [PCr] fall during constant work rate knee extension took 50 % longer and the critical power was 10 % higher compared with normoxia—though the terminal values of [PCr] and pH were the same . PCr recovery has been shown to be positively associated with age  again giving an indication that at a similar [PCr] children recover quicker than adults.
Few studies have investigated sham or placebo affects relative to CWI—as of course cooling is difficult to blind to. The placebo effect is a well-accepted phenomenon within medicine and also influences sport performance , and has a potentially long-lasting effect . Utilising thermoneutral conditions with and without the addition of a placebo and CWI to measure recovery from 4 × 30 s sprints in male adults, Broatch and colleagues found that the placebo condition is as effective as CWI for the recovery of muscle strength despite there being no difference in blood markers . The absence of a difference indicates that perception of recovery with such modalities might have a larger effect than any physiological effect per se.
Cardiovascular Implications for cold-water immersion
Partial and head-out immersion can alter heart activity [108, 137–139] as well as restore central blood volume and enhance cardiac pre-load—though it has been suggested that a large proportion of the body mass needs exposed for cardiovascular changes to occur . Within an adolescent population it has been shown that CWI may normalize [138, 140] or even enhance parasympathetic heart rate modulation and restore vagal tone following intense exercise similar to the mechanism in an adult population . However, to date, the information on the implications of CWI on adolescent athletes is somewhat limited.
Exercise and CWI alter the hormonal milieu of the body and in turn modulate blood flow, fluid balance, heart rate and breathing frequency among other physiological parameters. Marked increases in the circulating levels of dopamine and noradrenaline with no concomitant change in cortisol have been found after immersion for 1 h in 14 °C water. This suggests that prolonged (1 h) head out immersion in such temperature is capable of triggering marked responses in the adrenergic system but limited effects on the hypothalamus-hypophysis axis in young males (22.2 ± 2.4 years), however the physiological significance of such marked acute changes is unknown .
Review and meta-analysis methods
In order to quantify the effectiveness of this recovery modality on various outcomes in young athletic populations, a computerized literature search was conducted, ending in February 2015, using Medline, SportDiscus, and ISI Web of Knowledge. The following key phrases and their combinations were used: cold, immersion, ice, water, recovery, training, adaptation, youth, elite, sport, exercise and child*. Reference lists of all articles were examined for identification of further eligible studies. 259 articles were identified from the initial literature search. Of these 133 were discarded as not being directly relevant to the area. 19 additional articles were identified from the reference lists of appropriate studies.
Priority was given to articles dealing with adolescent athletes (where the average age of the study participants were ≤20 years). This meant there were 17 articles included in this systematic review. We also focused on immediate use of cold-water therapies rather than delayed interactions.
Assessment of study quality
Study inclusion and exclusion criteria
Studies meeting the following criteria were considered for review: (1) The study design was randomised into an intervention group and a control group; (2) only outcome variables measured at 24 h or less or 96 h or greater post exercise were included; (3) outcome measures were taken longitudinally and not simply pre and post a chronic training period or serial recovery interventions; (4) the intervention was applied within 1 h post exercise and permitted to be repeated on subsequent days and (5) participants could be male or female and of any athletic training status. Studies were excluded when (1) the control group received an intervention with rationale to improve recovery; (2) the observed effect could not specifically be attributed to intervention group (e.g., CWI group received multiple interventions, such as active recovery and CWI); (3) there were insufficient data to report an effect size; (4) if the intervention happened pre exercise and (5) if no longitudinal data was reported for recovery.
All meta-analyses calculations were conducted using an open source software (Open Meta-Analyst, Center for Evidence-Based Medicine, Brown University School of Public Health, USA). The standardized mean differences were calculated (Hedges g) along with 95 % CI using data extracted from the manuscript to compare the intervention group to the control. If these values were only displayed in figures, then a commercially available software (Graph Digitizer v2.26, Datatrend Software, Raleigh, North Carolina, USA) was used to digitize the graphs and extract the means and standard deviations. Effect sizes of <0.2, <0.5, <0.8, >0.8 were considered trivial, small, moderate and large, respectively . The numbers of studies proved too small to differentiate findings between pre and post pubertal populations so the analysis was performed for all studies with participants under 20 years of age. As a common effect size cannot be assumed where we are combining the data under broader headings, the random-effects model for the meta-analysis of pooled data was implemented. Heterogeneity was assessed using the I2 statistic, which describes the percentage of variability in effect estimates that is due to heterogeneity rather than chance. I2 values of 25, 50 and 75 % respectively represent low, moderate and high statistical heterogeneity.
The outcome variables were grouped by type according to the observed effects. Within the ‘Physiological’ group we included measures of tympanic temperature, heart rate (HR), lactate (La), bicarbonate (HCO3), creatine kinase (CK) and lactate dehydrogenase (LDH) where appropriate. We utilized fatigue, delayed onset muscle soreness (DOMS) and total quality recovery scores (TQR) scores in the ‘subjective’ category and then clustered performance measures of ‘power’ and ‘endurance’ (vertical jump (VJ), squat jump (SJ), countermovement jump (CMJ), peak power (PP), speed, agility & maximal voluntary contraction (MVC) and repeated sprints, time trials, total distance and shuttle run scores respectively). We then categorized these as immediate or delayed effects (immediate being <24 h and delayed >96 h).
Results of the meta-analysis: overall effects of water immersion on recovery in adolescent athletes
The published literature within an adolescent population encompasses 19 studies based on age criteria alone. One study was discounted as it utilized multiple interventions (combined sauna, CWI and jacuzzi) for recovery . One was discounted as the control group was active to promote recovery . Two were discounted as they utilized pre-cooling rather than cooling as a recovery intervention [146, 147], one for the absence of a control group  and two as they utilized chronic recovery interventions [149, 150]. This left 12 studies included in the review. Of those remaining, only 10 studies utilised CWI (Additional file 1: Table S1); [140, 151–158] and 6 studies utilised protocols alternating hot and cold-water immersion techniques [149, 151, 155, 159–161]. Of these studies five looked at the intervention versus a passive control [128, 140, 152–154], 3 against a thermoneutral immersion control [156–158] and the remaining four against each other and a passive control [151, 155, 159, 160]. Within these studies the outcome variables were diverse as well as the research design. Passive intervention was defined as either seated rest or no intervention. Across studies the main differences came in temperature, immersion depth and total time (Additional file 1: Table S1). The CWI ranged from 8 to 15 °C in all of the studies (the majority being 10–15 °C with one under 10 °C). The total duration of immersion ranged from 5 to 15 min with a median value of 10, (mean = 10.14 ± 2.70 min). Five of the studies utilized a single immersion [128, 140, 151, 154, 158] with the remainder using repeated periods of immersion to reach the total time. In all of the 12 studies assessed we can consider the immersion periods to be short term (i.e. <15 min). The depth of immersion, when stated, ranged from the fibular head to the shoulders though most commonly this was somewhere within the thorax region with complete immersion of the lower limbs.
In terms of delayed effects, again there is limited support for using CWI in this population, though there may be a small effect on endurance outcomes (ES = 0.43, 95 % CI −0.13, 0.99) however the evidence comes from only 3 studies.
Quality of the evidence
The PEDro ratings indicated an average score of 4.17. This would suggest that the quality of the trials so far published is low to moderate. There appears a case for selection bias as only 2 studies utilized a concealed allocation suggesting that this is an area that can be addressed to improve the quality of studies in the area (Fig. 2). One of the main issues to be solved before reaching consensus in this area is the limitations and quality of the studies so far conducted. While this is easy to criticize, it is important to remind the readers that the design of proper randomized controlled studies is made challenging by the fact that individuals cannot be blind to the cold water intervention, however multiple interventions involving water immersion of different temperature could be used and for sure the scientists performing the measurements could and should be blind to the treatments when assessing its effects in order to reduce bias and improve the quality of the results presented. Finally, considering the relatively small sample sizes of the studies and the small effect sizes identified in this meta-analysis, it seems clear that larger scale studies are needed.
Short and long-term adaptation
Recent reviews have concluded that the literature suggests CWI may attenuate adaptations to training; however, due to the methodology employed further research investigating the effects on adaptation for athletic performance is required . Recent work in healthy young adults performing strength training programmes suggests that CWI can attenuate adaptations to training in comparison to active recovery over 12 weeks  and in comparison to simply not cooling over 6 weeks . These preliminary studies as well as previous work  indicate that rapid reduction in temperature with a consequent reduction in swelling and inflammation of the affected areas could impair the improvement in skeletal muscle form and function following resistance exercise programmes.
Therefore a cautious approach to the use of interventions aimed at reducing the exercise-induced inflammatory responses is needed and should be based on the desired outcome and the health, age and training status of the individual. In the case of young athletes, manipulations of training loads and exercise modalities should be sought to make sure that the progressions and loading patterns are adequate to the age and training experience and recovery strategies should not need to be employed to guarantee excessive loads.
It is unknown if CWI can be detrimental for adolescent athletes trying to increase strength and muscle hypertrophy as at the moment there is no study which has addressed this aspect in this population. We do not know much about the long-term effects of repeated exposures to CWI and so caution should be exercised when repeatedly using this modality in an adolescent population. While the use of this recovery modality may allow a faster recovery rate and also facilitate increased training volume and intensity one wonders if the same level of adaptation may occur without the use of CWI. For sure practitioners should always ask themselves if the intervention is appropriate for the training outcomes and if the risks outweigh the benefits.
Based on the findings from this review and meta-analysis it would appear that the effects of CWI on young athletes are small to non-existent either acutely or over a few days after the exercise bout (i.e. >96 h). The current literature provides only a small number of studies characterizing the acute and chronic responses to CWI and CWT in adolescent athletes (221 subjects in total across each condition) and as a consequence it is difficult to draw definite conclusions. While most protocols currently implemented in the field are based on findings on adult populations and suggest positive effect in reducing DOMS , no hindering of glycogen re-synthesis  and no attenuation in endurance capacity when applied during a training period  currently the knowledge is too limited regarding adolescent athletes with reference to muscle remodeling as a consequence of training.
It is clear that there needs to be a consensus reached on the temperature and methodologies employed and indeed there would appear to be a need for characterization of an individual’s response to both a training session and the use of recovery interventions. Cold itself is not likely to affect recovery from all types of exercise uniformly. CWI protocols of excessive duration may actually exacerbate the concentration of cytokines in circulation post-exercise . It may be that similar to hormesis there is a low-dose stimulation, high dose inhibition relationship.
This highlights the need to differentiate the use of CWI within training and/or competition, which is further complicated by the influence of growth and maturation in the adolescent athlete. It may be that due to their lower relative power young athletes cannot create enough ‘stress’, to need to recover from. Furthermore, preventing inflammation may actually hinder recovery  so in some cases it may be better to avoid using interventions to reduce the inflammatory responses to allow adaptation to training and consider CWI applications only when a high density of competitive events (i.e. competition phases), inducing DOMS are planned as such an approach might promote faster recovery.
Future studies should utilize modern non-invasive technologies to characterize current practices of coaches in relation to adolescent athletes. The use of minimally and non-invasive technologies could overcome the ethical issues around invasive methods in this population and provide practical tools to ascertain the efficacy and the need of CWI. Techniques like thermal imaging to detect blood flow and track the development of DOMS , tensiomyography and twitch interpolation techniques to assess evoked responses and salivary assays might provide a solution to understand more about the appropriateness of such recovery methods with a psychobiological approach as it seems clear that perception of recovery might be affected.
Recovery activities should be part of a well-planned, controlled and managed training programme. This is important not only for elite senior athletes but it is of particular relevance for young athletes involved in full time sports academies and/or engaged in structured long term development plans. The popularization of practices by the media means that protocols and procedures in place for elite senior athletes can be extended to adolescent performers without an appropriate knowledge of the risk and reward. CWI is a popular intervention used for “recovery” by many athletes/coaches. Asking the question “recovery from what?” before thinking about its use should be the first step before deciding if it is appropriate to use it. However, when young athletes (in particular pre- and peri-pubertal) are to be exposed to such practice, it is important to understand the time and the place for the use of CWI and most of all be aware of the current knowledge (or lack of it) about the implications for using such interventions. Recovery is a holistic process which should consider the acute implications of recovery strategies in the context of the long term outcomes of a training programme. So, when planning the use of recovery modalities, practitioners, coaches and parents should understand that physical training is characterized by stressors which stimulate our bodies to adapt. In the case of skeletal muscle, producing the final result of stronger and better functioning muscles. When intervening to accelerate such processes and/or amplify the potential for adaptation, we need to make sure that the intervention used is safe, effective, and it does not harm the long term potential for adaptation in a young athlete. According to our literature review and meta-analysis, more work is needed to ascertain the impact and effectiveness of cold applications on a pre or peri-pubertal population, so until more and better evidence becomes available the advice to young athletes can be the same that our ancestors suggested: “Perfer et obdura, dolor hic tibi proderit olim” (“Be patient and tough; someday this pain will be useful to you”. Ovid, Amores, Book III, Elegy XI).
Defined as age 10 to 19 by the United Nations (http://www.un.org/esa/socdev/documents/youth/fact-sheets/youth-definition.pdf).
contrast water therapy
delayed onset muscle soreness
exercise induced muscle damage
- HCO3 :
maximal voluntary contraction
physiotherapy evidence database
reactive oxygen species
total quality recovery
AM reviewed the literature, organized and wrote the initial article and conceptualized and compiled figures and tables. MC critically revised the article, contributed to the writing and the structure, consulted on the general structure and direction the article should take. Both authors read and approved the final manuscript.
AM is the Senior Sports Physiologist at Aspire Academy in Doha, Qatar. He was formerly the Senior Sports Physiologist at the sportscotland institute of sport where he prepared athletes for the Commonwealth and Olympic Games from 2009 to 2014. MC is the current Head of Sports Physiology at Aspire Academy in Doha, Qatar. Before joining Aspire, he was the Head of Sports Science and Research at the British Olympic Association and led the Sports Science activities for the preparation of Team GB at the 2008, 2010 and 2012 Olympic Games.
Compliance with ethical guidelines
Competing interests The authors declare that they have no competing interests.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Viru A. Molecular cellular mechanisms of training effects. J Sport Med Phys Fit. 1994;34:309–22.Google Scholar
- Mujika I: The influence of training characteristics and tapering on the adaptation in highly trained individuals: A review. Int J Spo Med. 1998;19(7):439–46.View ArticleGoogle Scholar
- Issurin VB. New horizons for the methodology and physiology of training periodization. Sport Med. 2010;40:189–206.View ArticleGoogle Scholar
- Matos N, Winsley RJ, Williams CA. Prevalence of nonfunctional overreaching/overtraining in young English athletes. Med Sci Sports Exerc. 2011;43:1287–94.PubMedView ArticleGoogle Scholar
- Nosaka K, Newton M. Difference in the magnitude of muscle damage between maximal and submaximal eccentric loading. J Strength Cond Res. 2002;16:202–8.PubMedGoogle Scholar
- Armstrong R. Initial events in exercise-induced muscular injury. Med Sci Sports Exerc. 1990;22:429–35.PubMedView ArticleGoogle Scholar
- Tidball JG. Inflammatory processes in muscle injury and repair. Am J Physiol Regul Integr Comp Physiol. 2005;288:R345–53.PubMedView ArticleGoogle Scholar
- Sorichter S, Puschendorf B, Mair J. Skeletal muscle injury induced by eccentric muscle action: muscle proteins as markers of muscle fiber injury. Ex Imm Rev. 1999;5:5–21.Google Scholar
- Nosaka K, Clarkson PM. Muscle damage following repeated bouts of high force eccentric exercise. Med Sci Sports Exerc. 1995;27:1263–9.PubMedView ArticleGoogle Scholar
- Barnett A. Using recovery modalities between training sessions in elite athletes: does it help? Sport Med. 2006;36:781–96.View ArticleGoogle Scholar
- Vaile J, Halson S, Gill N, Dawson B. Effect of hydrotherapy on recovery from fatigue. Int J Sports Med. 2008;29:539–44.PubMedView ArticleGoogle Scholar
- Gill N, Beaven C, Cook C. Effectiveness of post-match recovery strategies in rugby players. Br J Sports Med. 2006;40:260–3.PubMed CentralPubMedView ArticleGoogle Scholar
- Montgomery PG, Pyne D, Hopkins W, Dorman JC, Cook K, Minahan CL. The effect of recovery strategies on physical performance and cumulative fatigue in competitive basketball. J Sports Sci. 2008;26:1135–45.PubMedView ArticleGoogle Scholar
- Duffield R, Edge J, Merrells R, Hawke E, Barnes M, Simcock D, Gill N. The effects of compression garments on intermittent exercise performance and recovery on consecutive days. Int J Sports Physiol Perform. 2008;3:454–68.PubMedGoogle Scholar
- Bieuzen F, Bleakley C, Costello J. Contrast water therapy and exercise induced muscle damage: a systematic review and meta-analysis. PLoS One. 2013;8:e62356.PubMed CentralPubMedView ArticleGoogle Scholar
- Poppendieck W, Faude O, Wegmann M, Meyer T. Cooling and performance recovery of trained athletes: a meta-analytical review. Int J Sports Physiol Perform. 2013;8:227–42.PubMedGoogle Scholar
- Bleakley C, Bieuzen F, Davison GW, Costello JT. Whole-body cryotherapy: empirical evidence and theoretical perspectives. Open Access J Sport Med. 2014;5:25–36.View ArticleGoogle Scholar
- White GE, Wells GD. Cold-water immersion and other forms of cryotherapy: physiological changes potentially affecting recovery from high-intensity exercise. Extrem Physiol Med. 2013;2:26.PubMed CentralPubMedView ArticleGoogle Scholar
- Versey NG, Halson S, Dawson BT. Water immersion recovery for athletes: effect on exercise performance and practical recommendations. Sport Med. 2013;43:1101–30.View ArticleGoogle Scholar
- Cochrane DJ: Alternating hot and cold water immersion for athlete recovery: a review. Phys Ther Sport. 2004;5(1):26–32.View ArticleGoogle Scholar
- Leeder J, Gissane C, van Someren K, Gregson W, Howatson G. Cold water immersion and recovery from strenuous exercise: a meta-analysis. Br J Sports Med. 2012;46:233–40.PubMedView ArticleGoogle Scholar
- Clarkson P, Sayers SP. Etiology of exercise-induced muscle damage. Can J Appl Physiol. 1999;24:234–48.PubMedView ArticleGoogle Scholar
- Howatson G, van Someren K. The Prevention and treatment of exercise-induced muscle damage. Sport Med. 2008;38:483–503.View ArticleGoogle Scholar
- Jones D, Newham DJ, Round JM, Tolfree SE. Experimental human muscle damage: morphological changes in relation to other indices of damage. J Physiol. 1986;375:435–48.PubMed CentralPubMedView ArticleGoogle Scholar
- Newham DJ, Mills KR, Quigley BM, Edwards RH. Pain and fatigue after concentric and eccentric muscle contractions. Clin Sci. 1983;64:55–62.PubMedView ArticleGoogle Scholar
- Byrnes WC, Clarkson P, White JS, Hsieh SS, Frykman PN, Maughan R. Delayed onset muscle soreness following repeated bouts of downhill running. J Appl Physiol. 1985;59:710–5.PubMedGoogle Scholar
- Davies C, Barnes C. Negative (eccentric) work. I. Effects of repeated exercise. Ergonomics. 1972;15:3–14.PubMedGoogle Scholar
- Newham DJ, Jones D, Clarkson P. Repeated high-force eccentric exercise: effects on muscle pain and damage. J Appl Physiol. 1987;63:1381–6.PubMedGoogle Scholar
- McHugh MP. Recent advances in the understanding of the repeated bout effect: the protective effect against muscle damage from a single bout of eccentric exercise. Scand J Med Sci Sports. 2003;13:88–97.PubMedView ArticleGoogle Scholar
- Vanderthommen M, Soltani K. Does neuromuscular electrical stimulation influence muscle recovery after maximal isokinetic exercise ? Isokinet Exerc. 2007;15:143–9.Google Scholar
- Nosaka K, Newton M. Repeated eccentric exercise bouts do not exacerbate muscle damage and repair. J Strength Cond Res. 2002;16:117–22.PubMedGoogle Scholar
- Malm C. Exercise-induced muscle damage and inflammation: Fact or fiction? Acta Physiol Scand. 2001;171:233–9.PubMedView ArticleGoogle Scholar
- Clarkson P, Hubal MJ. Exercise-induced muscle damage in humans. Am J Phys Med Rehabil. 2002;81(11 Suppl):S52–69.PubMedView ArticleGoogle Scholar
- Smith LL, Fulmer MG, Holbert D, McCammon MR, Houmard JA, Frazer DD, Nsien E, McCammon RG. The impact of a repeated bout of eccentric exercise on muscular strength, muscle soreness and creatine kinase. Br J Sports Med. 1994;28:267–71.PubMed CentralPubMedView ArticleGoogle Scholar
- Ebbeling CB, Clarkson P. Exercise-induced muscle damage and adaptation. Sport Med. 1989;7:207–34.View ArticleGoogle Scholar
- Clarkson P, Tremblay I. Exercise-induced muscle damage, repair, and adaptation in humans. J Appl Physiol. 1988;65:1–6.PubMedGoogle Scholar
- Gulick DT, Kimura IF. Delayed onset muscle soreness: what is it and how do we treat it? J Sport Rehabil. 1996;5:234–43.Google Scholar
- Meeusen R, Lievens P. The use of cryotherapy in sports injuries. Sport Med. 1986;3:398–414.View ArticleGoogle Scholar
- Swenson C, Swärd L, Karlsson J. Cryotherapy in sports medicine. Scand J Med Sci Sports. 1996;6:193–200.PubMedView ArticleGoogle Scholar
- Merrick MA, Knight KL, Ingersoll CD, Potteiger JA. The effects of ice and compression wraps on intramuscular temperatures at various depths. J Athl Train. 1993;28:236–45.PubMed CentralPubMedGoogle Scholar
- Paddon-Jones DJ, Quigley BM. Effect of cryotherapy on muscle soreness and strength following eccentric exercise. Int J Sports Med. 1997;18:588–93.PubMedView ArticleGoogle Scholar
- Eston R, Peters D. Effects of cold water immersion on the symptoms of exercise-induced muscle damage. J Sports Sci. 1999;17:231–8.PubMedView ArticleGoogle Scholar
- Yanagisawa O, Niitsu M, Takahashi H, Goto K, Itai Y. Evaluations of cooling exercised muscle with MR imaging and 31P MR spectroscopy. Med Sci Sports Exerc. 2003;35:1517–23.PubMedView ArticleGoogle Scholar
- Sellwood KL, Brukner P, Williams D, Nicol A, Hinman R. Ice-water immersion and delayed-onset muscle soreness: a randomised controlled trial. Br J Sports Med. 2007;41:392–7.PubMed CentralPubMedView ArticleGoogle Scholar
- Falk B, Dotan R. Child-adult differences in the recovery from high-intensity exercise. Exerc Sport Sci Rev. 2006;34:107–12.PubMedView ArticleGoogle Scholar
- Marginson V, Rowlands AV, Gleeson NP, Eston RG. Comparison of the symptoms of exercise-induced muscle damage after an initial and repeated bout of plyometric exercise in men and boys. J Appl Physiol. 2005;99:1174–81.PubMedView ArticleGoogle Scholar
- Marginson V, Eston R. The relationship between torque and joint angle during knee extension in boys and men. J Sports Sci. 2001;19:875–80.PubMedView ArticleGoogle Scholar
- Marginson V, Eston R. Symptoms of exercise-induced muscle damage in boys and men following two bouts of eighty plyometric jumps. J Physiol. 2002;539:75.Google Scholar
- Eston R, Byrne C, Twist C. Muscle function after exercise-induced muscle damage: considerations for athletic performance in children and adults. J Exerc Sci Fit. 2003;1:85–96.Google Scholar
- Chen TC, Chen HL, Liu YC, Nosaka K. Eccentric exercise-induced muscle damage of pre-adolescent and adolescent boys in comparison to young men. Eur J Appl Physiol. 2014;114:1183–95.PubMedView ArticleGoogle Scholar
- Soares J, Mota P, Duarte J, Appell H: Children are less susceptible to exercise-induced muscle damage than adults: a preliminary investigation. Pediatr Exerc Sci. 1996;8(4):361–7.Google Scholar
- Duarte JA, Magalhães JF, Monteiro L, Almeida-Dias A, Soares JM, Appell HJ. Exercise-induced signs of muscle overuse in children. Int J Sports Med. 1999;20:103–8.PubMedView ArticleGoogle Scholar
- Bleakley C, Davison GW. What is the biochemical and physiological rationale for using cold-water immersion in sports recovery? A systematic review. Br J Sports Med. 2010;44:179–87.PubMedView ArticleGoogle Scholar
- Tee JC, Bosch AN, Lambert MI. Metabolic consequences of exercise-induced muscle damage. Sports Med. 2007;37:827–36.PubMedView ArticleGoogle Scholar
- Proske U, Morgan DL: Muscle damage from eccentric exercise: Mechanism, mechanical signs, adaptation and clinical applications. J Physiol. 2001;537:333–45.PubMed CentralPubMedView ArticleGoogle Scholar
- Vollaard NBJ, Shearman JP, Cooper CE. Exercise-induced oxidative stress:myths, realities and physiological relevance. Sports Med. 2005;35:1045–62.PubMedView ArticleGoogle Scholar
- Tong TK, Lin H, Lippi G, Nie J, Tian Y. Serum oxidant and antioxidant status in adolescents undergoing professional endurance sports training. Oxid Med Cell Longev. 2012;2012:741239.PubMed CentralPubMedView ArticleGoogle Scholar
- Kabasakalis A, Tsalis G, Zafrana E, Loupos D, Mougios V. Effects of endurance and high-intensity swimming exercise on the redox status of adolescent male and female swimmers. J Sports Sci. 2014;32:747–56.PubMedView ArticleGoogle Scholar
- MacIntyre DL, Reid WD, McKenzie DC. Delayed muscle soreness The inflammatory response to muscle injury and its clinical implications. Sport Med. 1995;20:24–40.View ArticleGoogle Scholar
- Pedersen B, Toft AD. Effects of exercise on lymphocytes and cytokines. Br J Sports Med. 2000;34:246–51.PubMed CentralPubMedView ArticleGoogle Scholar
- Smith LL. Acute inflammation: the underlying mechanism in delayed onset muscle soreness? Med Sci Sports Exerc. 1991;23:542–51.PubMedGoogle Scholar
- Fielding RA, Manfredi TJ, Ding W, Fiatarone MA, Evans WJ, Cannon JG. Acute phase response in exercise. III. Neutrophil and IL-1 beta accumulation in skeletal muscle. Am J Physiol. 1993;265:R166–72.PubMedGoogle Scholar
- Paulsen G, Mikkelsen UR, Raastad T, Peake JM. Leucocytes, cytokines and satellite cells: What role do they play in muscle damage and regeneration following eccentric exercise? Exerc Immunol Rev. 2012;18:42–97.PubMedGoogle Scholar
- Pedersen B. Exercise and cytokines. Immunol Cell Biol. 2000;78:532–5.PubMedView ArticleGoogle Scholar
- Smith LL, Anwar A, Fragen M, Rananto C, Johnson R, Holbert D. Cytokines and cell adhesion molecules associated with high-intensity eccentric exercise. Eur J Appl Physiol. 2000;82:61–7.PubMedView ArticleGoogle Scholar
- Hellsten Y, Frandsen U, Orthenblad N, Sjødin B, Richter EA. Xanthine oxidase in human skeletal muscle following eccentric exercise: a role in inflammation. J Physiol. 1997;498(1):239–48.PubMed CentralPubMedView ArticleGoogle Scholar
- Ostrowski K, Rohde T, Zacho M, Asp S, Pedersen B. Evidence that interleukin-6 is produced in human skeletal muscle during prolonged running. J Physiol. 1998;508:949–53.PubMed CentralPubMedView ArticleGoogle Scholar
- Croisier JL, Camus G, Venneman I, Deby-Dupont G, Juchmès-Ferir A, Lamy M, Crielaard JM, Deby C, Duchateau J. Effects of training on exercise-induced muscle damage and interleukin 6 production. Muscle Nerve. 1999;22:208–12.PubMedView ArticleGoogle Scholar
- Northoff H, Berg A. Immunologic mediators as parameters of the reaction to strenuous exercise. Int J Sports Med. 1991;12(Suppl 1):S9–15.PubMedView ArticleGoogle Scholar
- Reihmane D, Dela F: Interleukin-6: Possible biological roles during exercise. Eur J Sport Sci. 2014;14(3):37–41.View ArticleGoogle Scholar
- Ostrowski K, Hermann C, Bangash A, Schjerling P, Nielsen JN, Pedersen B. A trauma-like elevation of plasma cytokines in humans in response to treadmill running. J Physiol. 1998;513:889–94.PubMed CentralPubMedView ArticleGoogle Scholar
- Croft L, Bartlett JD, MacLaren DPM, Reilly T, Evans L, Mattey DL, Nixon NB, Drust B, Morton J. High-intensity interval training attenuates the exercise-induced increase in plasma IL-6 in response to acute exercise. Appl Physiol Nutr Metab. 2009;34:1098–107.PubMedView ArticleGoogle Scholar
- Mäestu J, Jürimäe J, Purge P, Rämson R, Jürimäe T. Performance improvement is associated with higher postexercise responses in interleukin-6 and tumor necrosis factor α concentrations. J Sports Med Phys Fitness. 2010;50:524–9.PubMedGoogle Scholar
- Eliakim A, Portal S, Zadik Z, Rabinowitz J, Adler-Portal D, Cooper DM, Zaldivar F, Nemet D. The effect of a volleyball practice on anabolic hormones and inflammatory markers in elite male and female adolescent players. J Strength Cond Res. 2009;23:1553–9.PubMedView ArticleGoogle Scholar
- Timmons BW, Tarnopolsky MA, Snider DP, Bar-Or O. Immunological changes in response to exercise: Influence of age, puberty, and gender. Med Sci Sports Exerc. 2006;38:293–304.PubMedView ArticleGoogle Scholar
- Nieman DC, Kernodle MW, Henson DA, Sonnenfeld G, Morton DS. The acute response of the immune system to tennis drills in adolescent athletes. Res Q Exerc Sport. 2000;71:403–8.PubMedView ArticleGoogle Scholar
- Nemet D, Mills PJ, Cooper DM. Effect of intense wrestling exercise on leucocytes and adhesion molecules in adolescent boys. Br J Sports Med. 2004;38:154–8.PubMed CentralPubMedView ArticleGoogle Scholar
- Nemet D, Pontello AM, Rose-Gottron C, Cooper DM. Cytokines and growth factors during and after a wrestling season in adolescent boys. Med Sci Sports Exerc. 2004;36:794–800.PubMedView ArticleGoogle Scholar
- Fournier M, Ricci J: Skeletal muscle adaptation in adolescent boys: sprint and endurance training and detraining. Med Sci Sports Exerc. 1981;14(6):453–6.View ArticleGoogle Scholar
- Eriksson BO, Gollnick PD, Saltin B. Muscle metabolism and enzyme activities after training in boys 11–13 years old. Acta Physiol Scand. 1973;87:485–97.PubMedView ArticleGoogle Scholar
- Eriksson BO. Physical training, oxygen supply and muscle metabolism in 11–13-year old boys. Acta Physiol Scand Suppl. 1972;384:1–48.PubMedGoogle Scholar
- Gorianovas G, Skurvydas A, Streckis V, Brazaitis M, Kamandulis S, McHugh MP: Repeated bout effect was more expressed in young adult males than in elderly males and boys. Biomed Res Int. 2013;2013:1–10.View ArticleGoogle Scholar
- MacKelvie KJ, Khan KM, McKay HA. Is there a critical period for bone response to weight-bearing exercise in children and adolescents? a systematic review. Br J Sports Med. 2002;36(February):250–7.PubMed CentralPubMedView ArticleGoogle Scholar
- Cooper DM, Nemet D, Galassetti P. Exercise, stress, and inflammation in the growing child: from the bench to the playground. Curr Opin Pediatr. 2004;16:286–92.PubMedView ArticleGoogle Scholar
- Koch A, Pereira R, Machado M. The creatine kinase response to resistance exercise. J Musculoskelet Neuronal Interact. 2014;14:68–77.PubMedGoogle Scholar
- Webber L, Byrnes WWC, Rowland TTW, Foster V. Serum creatine kinase activity and delayed onset muscle soreness in prepubescent children: a preliminary study. Pediatr Exerc Sci. 1989;1:351–9.Google Scholar
- Baird MF, Graham SM, Baker JS, Bickerstaff GF: Creatine-kinase- and exercise-related muscle damage implications for muscle performance and recovery. J Nutr Metab. 2012;2012. doi:10.1155/2012/960363.
- Fournier S, Taffé P, Radovanovic D, Von Elm E, Morawiec B, Stauffer J-C, Erne P, Beggah A, Monney P, Pascale P, Iglesias J-F, Eeckhout E, Muller O. Myocardial Infarct size and mortality depend on the time of day—a large multicenter study. PLoS One. 2015;10:e0119157.PubMed CentralPubMedView ArticleGoogle Scholar
- Kopp J, Loos B, Spilker G, Horch RE. Correlation between serum creatinine kinase levels and extent of muscle damage in electrical burns. Burns. 2004;30:680–3.PubMedView ArticleGoogle Scholar
- Kaczor JJ, Ziolkowski W, Popinigis J, Tarnopolsky MA. Anaerobic and aerobic enzyme activities in human skeletal muscle from children and adults. Pediatr Res. 2005;57:331–5.PubMedView ArticleGoogle Scholar
- Gollnick PD, Armstrong R, Saubert CW, Piehl K, Saltin B. Enzyme activity and fiber composition in skeletal muscle of untrained and trained men. J Appl Physiol. 1972;33:312–9.PubMedGoogle Scholar
- Lexell J, Sjöström M, Nordlund AS, Taylor CC. Growth and development of human muscle: a quantitative morphological study of whole vastus lateralis from childhood to adult age. Muscle Nerve. 1992;15:404–9.PubMedView ArticleGoogle Scholar
- Bell RD, MacDougall JD, Billeter R, Howald H. Muscle fiber types and morphometric analysis of skeletal msucle in six-year-old children. Med Sci Sports Exerc. 1980;12:28–31.PubMedView ArticleGoogle Scholar
- Bouchard C, Lesage R, Lortie G, Simoneau JA, Hamel P, Boulay MR, Pérusse L, Thériault G, Leblanc C. Aerobic performance in brothers, dizygotic and monozygotic twins. Med Sci Sports Exerc. 1986;18:639–46.PubMedView ArticleGoogle Scholar
- McHugh MP, Pasiakos S. The role of exercising muscle length in the protective adaptation to a single bout of eccentric exercise. Eur J Appl Physiol. 2004;93:286–93.PubMedView ArticleGoogle Scholar
- McHugh MP, Tetro DT. Changes in the relationship between joint angle and torque production associated with the repeated bout effect. J Sports Sci. 2003;21:927–32.PubMedView ArticleGoogle Scholar
- Blascovich J, Mendes WB. Social psychophysiology and embodiment. In Handbook of: Social Psychology; 2010. p. 194–227.Google Scholar
- Blascovich J, Tomaka J. The biopsychosocial model of arousal regulation. Adv Exp Soc Psychol. 1996;28:1–51.View ArticleGoogle Scholar
- Leknes S, Berna C, Lee MC, Snyder GD, Biele G, Tracey I. The importance of context: When relative relief renders pain pleasant. Pain. 2013;154:402–10.PubMed CentralPubMedView ArticleGoogle Scholar
- Bastian B, Jetten J, Fasoli F. Cleansing the soul by hurting the flesh: the guilt-reducing effect of pain. Psychol Sci. 2011;22:334–5.PubMedView ArticleGoogle Scholar
- Low J, Reed A: Electrotherapy Explained: Principles and Practice. 2000.
- Tipton MJ. The concept of an “Integrated Survival System” for protection against the responses associated with immersion in cold water. J R Nav Med Serv. 1993;79:11–4.PubMedGoogle Scholar
- Morton J, Kayani AC, McArdle A, Drust B: The Exercise-Induced stress response of skeletal muscle, with specific emphasis on humans. Sports Med. 2009;39:643–62.PubMedView ArticleGoogle Scholar
- Egan B, Zierath JR. Exercise metabolism and the molecular regulation of skeletal muscle adaptation. Cell Metab. 2013;17:162–84.PubMedView ArticleGoogle Scholar
- Brentano MA, Martins Kruel LF. A review on strength exercise-induced muscle damage: applications, adaptation mechanisms and limitations. J Sports Med Phys Fitness. 2011;51:1–10.PubMedGoogle Scholar
- Ferraro E, Giammarioli AM, Chiandotto S, Spoletini I, Rosano G. Exercise-induced skeletal muscle remodeling and metabolic adaptation: redox signaling and role of autophagy. Antioxid Redox Signal. 2014;00:1–23.Google Scholar
- Bassel-Duby R, Olson EN. Signaling pathways in skeletal muscle remodeling. Annu Rev Biochem. 2006;75:19–37.PubMedView ArticleGoogle Scholar
- Srámek P, Simecková M, Janský L, Savlíková J, Vybíral S. Human physiological responses to immersion into water of different temperatures. Eur J Appl Physiol. 2000;81:436–42.PubMedView ArticleGoogle Scholar
- Bonde-Petersen F, Schultz-Pedersen L, Dragsted N. Peripheral and central blood flow in man during cold, thermoneutral, and hot water immersion. Aviat Sp Env Med. 1992;63:346–50.Google Scholar
- Park KS, Choi JK, Park YS. Cardiovascular regulation during water immersion. Appl Hum Sci. 1999;18:233–41.View ArticleGoogle Scholar
- Wilcock IM, Cronin J, Hing WA. Physiological response to water immersion: a method for sport recovery? Sports Med. 2006;36:747–65.PubMedView ArticleGoogle Scholar
- Knight KL, Londeree BR. Comparison of blood flow in the ankle of uninjured subjects during therapeutic applications of heat, cold, and exercise. Med Sci Sports Exerc. 1980;12:76–80.PubMedView ArticleGoogle Scholar
- DeGroot DW, Gallimore RP, Thompson SM, Kenefick RW. Extremity cooling for heat stress mitigation in military and occupational settings. J Therm Biol. 2013;38:305–10.View ArticleGoogle Scholar
- Gregson W, Black MA, Jones H, Milson J, Morton J, Dawson BT, Atkinson G, Green DJ. Influence of cold water immersion on limb and cutaneous blood flow at rest. Am J Sports Med. 2011;39:1316–23.PubMedView ArticleGoogle Scholar
- Foldes FF, Kuze S, Vizi ES, Deery A. The influence of temperatute on neuromuscular performance. J Neural Transm. 1978;43:27–45.PubMedView ArticleGoogle Scholar
- Ghaderi F, Banakar S, Rostami S. Effect of pre-cooling injection site on pain perception in pediatric dentistry: “a randomized clinical trial”. Dent Res J (Isfahan). 2013;10:790–4.Google Scholar
- Algafly AA, George KP. The effect of cryotherapy on nerve conduction velocity, pain threshold and pain tolerance. Br J Sports Med. 2007;41:365–9.PubMed CentralPubMedView ArticleGoogle Scholar
- Ernst E, Fialka V. Ice freezes pain? A review of the clinical effectiveness of analgesic cold therapy. J Pain Symptom Manage. 1994;9:56–9.PubMedView ArticleGoogle Scholar
- Abramson DI, Chu LS, Tuck S, Lee SW, Richardson G, Levin M. Effect of tissue temperatures and blood flow on motor nerve conduction velocity. JAMA. 1966;198:1082–8.PubMedView ArticleGoogle Scholar
- Rutkove SB. Effects of temperature on neuromuscular electrophysiology. Muscle Nerve. 2001;24:867–82.PubMedView ArticleGoogle Scholar
- Janwantanakul P. The effect of quantity of ice and size of contact area on ice pack/skin interface temperature. Physiotherapy. 2009;95:120–5.PubMedView ArticleGoogle Scholar
- Glasgow PD, Ferris R, Bleakley C: Cold water immersion in the management of delayed-onset muscle soreness: Is dose important? A randomised controlled trial. Phys Ther Sport. 2014;15(4):228–33.PubMedView ArticleGoogle Scholar
- Peiffer JJ, Abbiss C, Watson G, Nosaka K, Laursen P. Effect of cold-water immersion duration on body temperature and muscle function. J Sports Sci. 2009;27:987–93.PubMedView ArticleGoogle Scholar
- Otte JW, Merrick MA, Ingersoll CD, Cordova ML. Subcutaneous adipose tissue thickness alters cooling time during cryotherapy. Arch Phys Med Rehabil. 2002;83:1501–5.PubMedView ArticleGoogle Scholar
- Myrer JW, Myrer KA, Measom GJ, Fellingham GW, Evers SL. Muscle temperature is affected by overlying adipose when cryotherapy is administered. J Athl Train. 2001;36:32–6.PubMed CentralPubMedGoogle Scholar
- Yanagisawa O, Homma T, Okuwaki T, Shimao D, Takahashi H. Effects of cooling on human skin and skeletal muscle. Eur J Appl Physiol. 2007;100:737–45.PubMedView ArticleGoogle Scholar
- Enwemeka CS, Allen C, Avila P, Bina J, Konrade J, Munns S. Soft tissue thermodynamics before, during, and after cold pack therapy. Med Sci Sports Exerc. 2002;34:45–50.PubMedView ArticleGoogle Scholar
- Rupp KA, Selkow NM, Parente WR, Ingersoll CD, Weltman AL, Saliba SA. The effect of cold water immersion on 48-hour performance testing in collegiate soccer players. J Strength Cond Res. 2012;26:2043–50.PubMedView ArticleGoogle Scholar
- Gregson W, Allan R, Holden S, Phibbs P, Doran D, Campbell I, Waldron S, Joo CH, Morton J. Postexercise cold-water immersion does not attenuate muscle glycogen resynthesis. Med Sci Sports Exerc. 2013;45:1174–81.PubMedView ArticleGoogle Scholar
- Yanagisawa O, Niitsu M, Yoshioka H, Goto K, Kudo H, Itai Y. The use of magnetic resonance imaging to evaluate the effects of cooling on skeletal muscle after strenuous exercise. Eur J Appl Physiol. 2003;89:53–62.PubMedView ArticleGoogle Scholar
- Shepherd JT, Rusch NJ, Vanhoutte PM. Effect of cold on the blood vessel wall. Gen Pharmacol. 1983;14:61–4.PubMedView ArticleGoogle Scholar
- Vanhatalo A, Fulford J, DiMenna FJ, Jones AM. Influence of hyperoxia on muscle metabolic responses and the power-duration relationship during severe-intensity exercise in humans: a 31P magnetic resonance spectroscopy study. Exp Physiol. 2010;95:528–40.PubMedView ArticleGoogle Scholar
- Fleischman A, Makimura H, Stanley TL, McCarthy M, Kron M, Sun N, Chuzi S, Hrovat MI, Systrom DM, Grinspoon SK. Skeletal muscle phosphocreatine recovery after submaximal exercise in children and young and middle-aged adults. J Clin Endocrinol Metab. 2010;95:E69–74.PubMed CentralPubMedView ArticleGoogle Scholar
- Beedie CJ, Foad AJ. The placebo effect in sports performance: a brief review. Sports Med. 2009;39:313–29.PubMedView ArticleGoogle Scholar
- Benedetti F, Pollo A, Colloca L. Opioid-mediated placebo responses boost pain endurance and physical performance: is it doping in sport competitions? J Neurosci. 2007;27:11934–9.PubMedView ArticleGoogle Scholar
- Broatch JR, Petersen A, Bishop DJ: Postexercise Cold-Water Immersion Benefits Are Not Greater than the Placebo Effect. Med Sci Sports Exerc 2014;46(11):2139–47.PubMedView ArticleGoogle Scholar
- Al Haddad H, Laursen PB, Chollet D, Lemaitre F, Ahmaidi S, Buchheit M. Effect of cold or thermoneutral water immersion on post-exercise heart rate recovery and heart rate variability indices. Auton Neurosci Basic Clin. 2010;156:111–6.View ArticleGoogle Scholar
- Al Haddad H, Parouty J, Buchheit M. Effect of daily cold water immersion on heart rate variability and subjective ratings of well-being in highly trained swimmers. Int J Sports Physiol Perform. 2012;7:33–8.PubMedGoogle Scholar
- Tipton MJ, Eglin CM, Golden FSC. Habituation of the initial responses to cold water immersion in humans: a central or peripheral mechanism? J Physiol. 1998;512:621–8.PubMed CentralPubMedView ArticleGoogle Scholar
- Parouty J, AlHaddad H, Quod M, Leprêtre PM, Ahmaidi S, Buchheit M. Effect of cold water immersion on 100-m sprint performance in well-trained swimmers. Eur J Appl Physiol. 2010;109:483–90.PubMedView ArticleGoogle Scholar
- Buchheit M, Peiffer JJ, Abbiss CR, Laursen PB. Effect of cold water immersion on postexercise parasympathetic reactivation. Am J Physiol Heart Circ Physiol. 2009;296:H421–7.PubMedView ArticleGoogle Scholar
- De Morton NA. The PEDro scale is a valid measure of the methodological quality of clinical trials: a demographic study. Aust J Physiother. 2009;55:129–33.PubMedView ArticleGoogle Scholar
- Cohen J. A power primer. Psychol Bull. 1992;112:155–9.PubMedView ArticleGoogle Scholar
- Buchheit M, Horobeanu C, Mendez-Villanueva A, Simpson B, Bourdon P. Effects of age and spa treatment on match running performance over two consecutive games in highly trained young soccer players. J Sports Sci. 2011;29:591–8.PubMedView ArticleGoogle Scholar
- Hamlin MJ. The effect of contrast temperature water therapy on repeated sprint performance. J Sci Med Sport. 2007;10:398–402.PubMedView ArticleGoogle Scholar
- Cross K, Wilson R, Perrin D: Functional performance following an ice immersion to the lower extremity. J Athl Train. 1996;31(2):113–6.PubMed CentralPubMedGoogle Scholar
- Duffield R: The use of mixed-method, part-body pre-cooling procedures for team-sport athletes training in the heat. J Strength Cond Res. 2009;23(9):2524–32.PubMedView ArticleGoogle Scholar
- Patterson SM, Udermann BE, Doberstein ST, Reineke DM. The effects of cold whirlpool on power, speed, agility, and range of motion. J Sports Sci Med. 2008;7:387–94.PubMed CentralPubMedGoogle Scholar
- Higgins T, Cameron M, Climstein M: Evaluation of passive recovery, cold water immersion, and contrast baths for recovery, as measured by game performances markers, between two simulated games of rugby union. J Strength Cond Res. 2012. doi:10.1519/JSC.0b013e31825c32b9.
- Higgins T: A random control trial of contrast baths and ice baths for recovery during competition in U/20 rugby union. J Strength Cond Res. 2011;25(4):1046–51.PubMedView ArticleGoogle Scholar
- De Nardi M, La Torre A, Barassi A, Ricci C, Banfi G: Effects of cold-water immersion and contrast-water therapy after training in young soccer players. J Sport Med Phys Fit. 2011;52(4):609–15.Google Scholar
- Higgins T: Evaluation of hydrotherapy, using passive tests and power tests, for recovery across a cyclic week of competitive rugby union. J Strength Cond Res. 2013;27(4):954–65.PubMedView ArticleGoogle Scholar
- Higgins T. Acute response to hydrotherapy after a simulated game of rugby. J Strength Cond Res. 2013;27:2851–60.PubMedView ArticleGoogle Scholar
- Jakeman JR, Macrae R, Eston R. A single 10-min bout of cold-water immersion therapy after strenuous plyometric exercise has no beneficial effect on recovery from the symptoms of exercise-induced muscle damage. Ergonomics. 2009;52:456–60.PubMedView ArticleGoogle Scholar
- King M, Duffield R. The effects of recovery interventions on consecutive days of intermittent sprint exercise. J Strength Cond Res. 2009;23:1795–802.PubMedView ArticleGoogle Scholar
- Rowsell GJ, Coutts AJ, Reaburn P, Hill-Haas S. Effect of post-match cold-water immersion on subsequent match running performance in junior soccer players during tournament play. J Sports Sci. 2011;29:1–6.PubMedView ArticleGoogle Scholar
- Rowsell GJ, Coutts AJ, Reaburn P, Hill-Haas S. Effects of cold-water immersion on physical performance between successive matches in high-performance junior male soccer players. J Sports Sci. 2009;27:565–73.PubMedView ArticleGoogle Scholar
- Ascensão A, Leite M, Rebelo AN, Magalhäes S, Magalhäes J. Effects of cold water immersion on the recovery of physical performance and muscle damage following a one-off soccer match. J Sports Sci. 2011;29:217–25.PubMedView ArticleGoogle Scholar
- Sayers MG, Calder AM, Sanders JG. Effect of whole-body contrast-water therapy on recovery from intense exercise of short duration. Eur J Sport Sci. 2011;11:293–302.View ArticleGoogle Scholar
- Kinugasa T, Kilding AE. A comparison of post-match recovery strategies in youth soccer players. J Strength Cond Res. 2009;23:1402–7.PubMedView ArticleGoogle Scholar
- Higgins T, Climstein M, Cameron M: Evaluation of hydrotherapy, using passive tests and power tests, for recovery across a cyclic week of competitive rugby union. J Strength Cond Res. 2012;27(4):954–65.View ArticleGoogle Scholar
- Roberts LA, Nosaka K, Coombes JS, Peake JM: Cold water immersion enhances recovery of submaximal muscle function following resistance exercise. Am J Physiol Regul Integr Comp Physiol 2014;307(8):R998–1006.PubMedView ArticleGoogle Scholar
- Yamane M, Ohnishi N, Matsumoto T. Does regular post-exercise cold application attenuate trained muscle adaptation? Int J Sports Med. 2015;36:647–53.PubMedView ArticleGoogle Scholar
- Yamane M, Teruya H, Nakano M, Ogai R, Ohnishi N, Kosaka M. Post-exercise leg and forearm flexor muscle cooling in humans attenuates endurance and resistance training effects on muscle performance and on circulatory adaptation. Eur J Appl Physiol. 2006;96:572–80.PubMedView ArticleGoogle Scholar
- Halson SL, Bartram J, West N, Stephens J, Argus CK, Driller MW, Sargent C, Lastella M, Hopkins WG, Martin DT: Does Hydrotherapy Help or Hinder Adaptation to Training in Competitive Cyclists? Med Sci Sports Exerc. 2014;46(8):1631–9.PubMedView ArticleGoogle Scholar
- Urso M. Anti-inflammatory interventions and skeletal muscle injury: benefit or detriment? J Appl Physiol. 2013;2013:920–8.View ArticleGoogle Scholar
- Al-Nakhli HH, Petrofsky JS, Laymon MS, Berk LS: The use of thermal infra-red imaging to detect delayed onset muscle soreness. J Vis Exp. 2012:59. doi:10.3791/3551.